Journal: Analytical and Bioanalytical Chemistry

Article Title: Interlaboratory assessment of quantification of SARS-CoV-2 RNA by reverse transcription digital PCR

doi: 10.1007/s00216-021-03680-2

Figure Lengend Snippet: Summary of the RT-dPCR platforms and methods of the participants

Article Snippet: Laboratory 10 used one-step RT-dPCR mix of both Bio-Rad (for E gene) and Sniper (for ORF 1ab and N gene), because no positive signals were observed for E gene using Sniper’s mix but normal positive and negative signals were obtained when changing the Sniper mix to Bio-Rad mix.

Techniques:

Journal: Analytical and Bioanalytical Chemistry

Article Title: Interlaboratory assessment of quantification of SARS-CoV-2 RNA by reverse transcription digital PCR

doi: 10.1007/s00216-021-03680-2

Figure Lengend Snippet: Participants’ results for copy number concentration of SARS-CoV-2 RNA ORF 1ab gene, E gene, and N gene. Results of the five laboratories using dPCR platforms of Bio-Rad are indicated with red circle, and results of the five laboratories using other platforms with blue diamond. Solid and dashed horizontal lines indicate RV and expanded uncertainty (95% level of confidence, k = 2) of each gene

Article Snippet: Laboratory 10 used one-step RT-dPCR mix of both Bio-Rad (for E gene) and Sniper (for ORF 1ab and N gene), because no positive signals were observed for E gene using Sniper’s mix but normal positive and negative signals were obtained when changing the Sniper mix to Bio-Rad mix.

Techniques: Concentration Assay